Photo-induced degradation of some flavins in aqueous solution |
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| Institution: | 1. Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, Pakistan;2. HEJ Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan;1. Department of Biochemistry, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, Thailand;2. Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, Thailand;3. School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, Thailand |
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| Abstract: | The blue-light induced photo-degradation of FMN, FAD, riboflavin, lumiflavin, and lumichrome in aqueous solution at pH 8 is studied by measurement of absorption coefficient spectral changes due to continuous excitation at 428 nm. The quantum yields of photo-degradation determined are ?D(riboflavin, pH 8) ≈ 7.8 × 10?3, ?D(FMN, pH 5.6) ≈ 7.3 × 10?3, ?D(FMN, pH 8) ≈ 4.6 × 10?3, ?D(FAD, pH 8) ≈ 3.7 × 10?4, ?D(lumichrome, pH 8) ≈ 1.8 × 10?4, and ?D(lumiflavin, pH 8) ? 1.1 × 10?5. In a mass-spectroscopic analysis, the photo-products of FMN dissolved in water (solution pH is 5.6) were identified to be lumichrome and the lumiflavin derivatives dihydroxymethyllumiflavin, formyllumiflavin, and lumiflavin-hydroxy-acetaldehyde. An absorption and emission spectroscopic characterisation of the primary photoproducts of FMN at pH 8 is carried out. |
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