Electrophoretically mediated microanalysis |
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Authors: | Nováková Sona Van Dyck Sigrid Van Schepdael Ann Hoogmartens Jos Glatz Zdenĕk |
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Institution: | Laboratory for Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, K. U. Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium. |
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Abstract: | This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn. |
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