Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas |
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Authors: | Hong Kyung Choi Jeong Ho Chang Il Hwan Ko Bong Yong Jeong Jung Bae Kim |
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Institution: | a Korea Institute of Ceramic Engineering and Technology, Seoul 153-801, Republic of Korea b Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea |
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Abstract: | This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state 29Si and 13C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process. |
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Keywords: | Functionalized mesoporous silicas DNA separation Electrostatic interaction Solid-state NMR Confocal microscope |
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