| 高效毛细管电泳紫外检测器光路的特殊性及其优化 |
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| 引用本文： | 许旭,吴如金,林炳承.高效毛细管电泳紫外检测器光路的特殊性及其优化[J].高等学校化学学报,1998,19(3):379-381. |
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| 作者姓名： | 许旭 吴如金 林炳承 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所, 大连, 116023;
2. 中国药科大学, 南京, 210009 |
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| 摘 要： | 紫外检测器用于高效毛细管电泳（ＨＰＣＥ）的主要难题在光路中，ＨＰＣＥ中熔融石英毛细管（内径通常在５０μｍ左右）直接放在光路中，一方面光程短，另一方面，光在透镜及毛细管界面上的折射与反射使光程常比毛细管内径短，甚至可能绕过毛细管内腔［１，２］．本文编制...
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| 关 键 词： | 高效毛细管电泳仪 紫外检测器 光路 灵敏度 |
| 收稿时间： | 1997-02-03 |
Studies on Ray-tracing of UV Detector in High Performance Capillary Electrophoresis |
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| XU Xu,WU Ru-Jin,LIN Bing-Cheng.Studies on Ray-tracing of UV Detector in High Performance Capillary Electrophoresis[J].Chemical Research In Chinese Universities,1998,19(3):379-381. |
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| Authors: | XU Xu WU Ru-Jin LIN Bing-Cheng |
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| Institution: | 1. Dalian Institute of Chemical Physics, Dalian, 116012;
2. China Pharmaceutical University, Nanjing, 210009 |
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| Abstract: | A computer program is compiled to work out the ray tracing of UV detector of high performance capillary Electrophoresis (HPCE). Based on calculating results obtained by calculating, some practical methods for adjusting the distance between lens and capillary are provided to make UV detector work on the optimatic condition. The experiments with the aid of the method show a satisfactory detection sensitivity. The method for adjusting ray tracing can be used into all kinds of HPCE UV detector. |
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| Keywords: | High performance capillary electrophoresis (HPCE) UV detector Ray tracing Sensitivity |
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