PICOSECOND FLUORESCENCE SPECTROSCOPY ON INCORPORATION PROCESSES OF HEMATOPORPHYRIN DERIVATIVE INTO MALIGNANT TUMOR CELLS in vitro |
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| Authors: | MIKIO YAMASHITA TAKAHISA TOMONO SHYUNSUKE KOBAYASHI KENJI TORIZUKA KATSUO AIZAWA TAKUZO SATO |
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| Institution: | Laser Research Section, Radio- and Opto-Electronics Division, Electrotechnical Laboratory, Umezono, Sakura, Niihari, Ibaraki 305, Japan;Department of Physiology, Tokyo Medical College, Shinjuku, Tokyo 160, Japan |
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| Abstract: | Abstract— By using a highly sensitive streak-camera technique, we investigate incorporation processes of HpD into malignant tumor m-KSA cells in vitro. The picosecond decays of the total fluorescence spectra, the wavelength-resolved fluorescence decays and the time-resolved fluorescence spectra from HpD in the cells are measured as a function of the incubation time. The results show that the aggregate component of HpD which has a fast fluorescence lifetime of 100 ps and a red-shifted band of ∼ 660 nm selectively accumulates more and more in the cells with the increase of the incubation time. |
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