Capillary electrophoretic separation of humic substances using hydroxyethyl cellulose as a buffer additive and its application to characterization of humic substances in a river water sample |
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Authors: | Toru Takahashi Jun Kawana Hitoshi Hoshino |
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Institution: | (1) Division of Environmentally Benign Systems, Graduate School of Environmental Studies, Tohoku University, 20 Aoba, Aramaki, Aoba-ku, Sendai 980–8579, Japan |
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Abstract: | We have developed a concise tool for the investigation of the transition of humic substances in environmental water. The separation
of water-soluble humic substances was achieved rapidly and effectively by capillary electrophoresis using a polyacrylamide-coated
capillary and a phosphate electrophoretic buffer solution (pH 7.0) containing hydroxyethyl cellulose. The separation mechanism
was assessed using the ultrafiltration technique. The effect of the complexation of humic substances with metal ions was studied
by using the proposed method. When Fe(III) ions or EDTA was added to the sample solution of fulvic acid, a distinct change
in the electropherogram pattern based on the conformational change of fulvic acid was observed. The successful application
of the proposed method to the characterization of humic substances in a river water sample was also demonstrated.
Figure Addition of Fe(III) ions or EDTA to a solution containing fulvic acid (FA) results in a distinct change in the electropherogram
pattern, which reflects the conformational change of FA: this forms the basis for the characterization of humic substances
in river water samples |
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Keywords: | Humic substances Capillary electrophoresis Metal ions Characterization Complexation |
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