Flow injection system with in-line Winkler's procedure using 16-way valve and spectrofluorimetric determination of dissolved oxygen in environmental waters |
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Authors: | Sakai Tadao Piao Shanji Teshima Norio Kuroishi Tadafumi Grudpan Kate |
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Institution: | a Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyata 470-0392, Japan b Naka Instruments Co., 795-27, Horiguchi, Hitachinaka, Ibaraki 312-8504, Japan c Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand |
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Abstract: | Flow injection spectrofluorimetry with in-line Winklers procedure was developed for the dissolved oxygen (DO) determination. 2-Thionaphthol reacted with iodine produced by Winkler’s method to form fluorescence inactive disulfide compound. To automate the process completely, a 5-channel flow system with a newly designed 16-way valve was assembled. The system consisted of a dispersion coil (DC), a precipitate formation coil (PFC), a precipitate dissolving coil (PDC), and extraction coil (EC). A calibration can be constructed by using a standard iodine solution for dissolved oxygen. The calibration graph was linear over the range 1.2×10−4∼6.0×10−4 mol l−1 iodine (1.96∼9.80 mg O l−1)). The relative standard deviation (n=6) was below 0.3% for the 4×10−4 mol l−1 iodine (6.27 mg O l−1) determination. The sample throughput was 12/h. |
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Keywords: | In-line reaction Flow injection analysis Winkler&rsquo s method 16-Way valve Fluorimetry 2-Thionaphthol Dissolved oxygen |
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