Determination of proline in wine using flow injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection |
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Authors: | Costin Jason W Barnett Neil W Lewis Simon W |
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Institution: | Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria 3217, Australia |
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Abstract: | Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10−6 M) and the detection limit was 1 × 10−8 M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy)33+ consumption and its associated effect on accuracy. |
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Keywords: | Tris(2 2&prime -bipyridyl)ruthenium(II) Chemiluminescence Ru(bipy)33+ Flow injection analysis Proline Wine |
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