| 应用量子点探针观察粒子辐射细胞损伤动态过程 |
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| 引用本文： | 杨林芳,鲁逸林,闫婧雯,吴李君,黄青.应用量子点探针观察粒子辐射细胞损伤动态过程[J].原子核物理评论,2014,31(1):96-100. |
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| 作者姓名： | 杨林芳 鲁逸林 闫婧雯 吴李君 黄青 |
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| 作者单位： | 中国科学院合肥物质科学研究院,合肥 230031; |
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| 基金项目： | 中国科学院百人计划项目(Y09BR12241);教育部留学回国人员启动基金项目;国家自然科学基金资助项目(11175204);中国科学院知识创新工程重要方向项目(KJCX2-YW-N34);国家重点基础研究发展计划项目(973计划)(2013CB934304)~~ |
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| 摘 要： | 荧光量子点由于具有发射荧光效率高、光稳定性好、无光漂白等优良特性,因此在生物学研究中得到广泛应用。用量子点标记细胞,通过量子点荧光可以对细胞进行长时程观察,实现活细胞动态示踪。利用量子点和DiO荧光染料对A549细胞膜双染,直观展示了量子点优于有机荧光染料的抗光漂白特性,并阐述了一种利用量子点对细胞A549进行标记并观测在α粒子辐照下细胞损伤动态变化过程的方法,这种方法对于阐明粒子辐射作用细胞的过程、了解辐射损伤的机理有一定的优势。
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| 关 键 词： | 荧光量子点 A549 细胞 粒子辐射 |
| 收稿时间： | 1900-01-01 |
Quantum Dots as a Probe to Observe the Process of Cell Damage Induced by Particle Irradiation |
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| YANG Linfang,LU Yilin,YAN Jingwen,WU Lijun,HUANG Qing.Quantum Dots as a Probe to Observe the Process of Cell Damage Induced by Particle Irradiation[J].Nuclear Physics Review,2014,31(1):96-100. |
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| Authors: | YANG Linfang LU Yilin YAN Jingwen WU Lijun HUANG Qing |
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| Institution: | Key Laboratory of Ion Beam Bio-engineering, Institute of Biotechnology and Agriculture Engineering,Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; |
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| Abstract: | Owing to the excellent optical properties such as high quantum yield of emission, photo-stability and anti-photobleaching, uorescent quantum dots(QDs) now have been extensively applied in bioscience research. Labeling cells by quantum dots allow long-term observation of the cellular processes. In this paper, we employed both QDs and DiO dye to label cells and thus showed the superior anti-photobleaching property of QDs directly, and also reported the method of application of QDs in observing the damage process of A549 cells induced by particle irradiation, demonstrating the advantage of application of QDs in the radiobiology research. |
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