| AFP抗体的标记及其电致化学发光免疫分析研究 |
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| 引用本文： | 庄惠生,王琼娥,陈国南,黄金陵,林培城.AFP抗体的标记及其电致化学发光免疫分析研究[J].高等学校化学学报,1999,20(8):1194-1199. |
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| 作者姓名： | 庄惠生 王琼娥 陈国南 黄金陵 林培城 |
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| 作者单位： | 1. 福州大学化学系, 福州 350002;
2. 福建省省立医院, 福州 350001 |
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| 基金项目： | 国家自然科学基金! (批准号 :2 9675 0 0 3)，国家教育部材料和生命过程分析科学开放研究实验室资助 |
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| 摘 要： | 用自制的异硫氰酸异鲁米诺标记AFP抗体,标记率平均为0.40;标记的抗体免疫活性和电致化学发光效率基本不变;研究了标记反应、双抗夹心免疫反应和电致化学发光反应的条件,建立了一种测定甲胎蛋白(α-fetoprotein,简称AFP)的双抗夹心电致化学发光免疫分析新方法,并用于测定人血清中的AFP,方法线性范围为5.0～100.0ngmL,检测限为2.0ngmL,本方法与放射免疫法比较,相关性良好.
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| 关 键 词： | 电致化学发光免疫分析(ECLIA) 异硫氰酸异鲁米诺 甲胎蛋白 标记 |
| 收稿时间： | 1998-09-14 |
Label of AFP Antibody and Electrochemiluminescence Immunoassay |
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| ZHUANG Hui-sheng,WANG Qiong-e,CHEN Guo-nan,HUANG Jin-ling,LIN Pei-Cheng.Label of AFP Antibody and Electrochemiluminescence Immunoassay[J].Chemical Research In Chinese Universities,1999,20(8):1194-1199. |
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| Authors: | ZHUANG Hui-sheng WANG Qiong-e CHEN Guo-nan HUANG Jin-ling LIN Pei-Cheng |
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| Institution: | 1. Department of Chemistry, Fuzhou University, Fuzhou 350002, China;
2. Provincial Capital Hospital of Fujian, Fuzhou 350001, China |
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| Abstract: | Anew chemiluminescent reagent isothiocyanato-isoluminol (ITCI) was used to label Anti-AFP. The molar incorporation ratios are estimated to be 0.25～0.50 mole of ITCI per mole Anti-AFP. The results show that there are no apparent changes in the immunoreactivity of the labeled Anti-AFPand the quantum efficiency of ITCIattached to the Anti-AFP. In addition, the conditions of labeling reaction,sandwich immunoassay and electrochemiluminescent reaction were studied. Morever, a new sandwich electrochemiluminescence immunoassay(ECLIA) is firstly established, and used to detect AFP in human serum. The calibration ranges is 5.0～100.0 ng/mLand the minimal detection concentration is 2.0 ng/mL. |
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| Keywords: | Electrochemiluminescence Immunoassay(ECLIA) Isothiocyanatoisoluminol α-Fetoprotein Label |
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