Determination of four nitroimidazole residues in poultry meat by liquid chromatography-mass spectrometry |
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Authors: | Hurtaud-Pessel D Delépine B Laurentie M |
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Affiliation: | Agence Fran?aise de Sécurité Sanitaire des Aliments, Laboratoire des Médicaments Vétérinaires, La Haute Marche, Javené, Fougères, France. |
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Abstract: | A multi-residue liquid chromatography-mass spectrometry (LC-MS) assay method is described for the determination of four nitroimidazoles in poultry muscle. The extraction procedure is based on liquid-liquid extraction with ethyl acetate followed by an evaporation step. A deuterated internal standard is used. The LC separation was made on a C18 bonded silica column with an aqueous formic acid (0.2%) solution-methanol-acetonitrile (81:13:6) mobile phase. Following electrospray ionization, the protonated molecular ion [M+H]+ is obtained for each compound. Monitoring several ions for each nitroimidazole provides the specificity required for confirmatory assay. Validation of the method was performed to estimate linearity, intra-day and inter-day repeatability, accuracy and detection limit. The present method is capable of identifying nitroimidazole residues in muscle at levels below 5 microg/kg. |
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