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Single Amino Acid Substitution Reveals Latent Photolyase Activity in Arabidopsis cry1 |
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| Authors: | Sarah Burney Ringo Wenzel Tilman Kottke Thomas Roussel Nathalie Hoang Jean-Pierre Bouly Robert Bittl Joachim Heberle Margaret Ahmad |
| Institution: | 1. PCMP, UR5, Université Paris?VI, Casier 156, 4 Place Jussieu, 75005 Paris (France);2. Freie Universit?t Berlin, AG Bittl, Arnimallee 14, 14195 Berlin (Germany);3. Department of Chemistry, Physical and Biophysical Chemistry, Universit?tsstrasse 25, 33615 Bielefeld (Germany);4. Freie Universit?t Berlin, Experimental Molecular Biophysics, Arnimallee 14, 14195 Berlin (Germany) |
| Abstract: | A quick switch: A single amino acid substitution at a conserved residue (D396N) of Arabidopsis cryptochrome-1 (Atcry1) confers single-stranded DNA repair activity in?vitro, conferring photolyase activity onto the cryptochrome. The mutant protein undergoes photoreduction of flavin to the fully reduced anionic form, similar to photolyases and unlike wild-type cryptochromes. |
| Keywords: | biophysics cryptochromes DNA damage flavin reduction photochemistry |
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