Effect of sodium dodecyl sulfate on lipase ofCandida lipolytica |
| |
Authors: | A E Nascimento G M Campos-Takaki |
| |
Institution: | (1) Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Campus Universitário, Cidade Universitária, Recife, Pernambuco, Brazil;(2) Departamento de Antibióticos, Universidade Federal de Pernambuco, Campus Universitário, Cidade Universitária, Recife, Pernambuco, Brazil |
| |
Abstract: | The effects of sodium dodecyl sulfate on extracellular lipase produced byCandida lipolytica have been studied. The microorganism was grown in culture medium containing different sodium dodecyl sulfate concentrations
added to the culture at different intervals of growth. The extracellular lipase activity was not detected when the treated
culture supernatants were directly tested in Yeast Mold Agar-Triolein-Rhodamine plates, regardless of surfactant addition
time and concentrations. However, after ammonium sulfate precipitation and dialysis, the extracellular lipase activity could
be recovered. Therefore, the surfactant, under the experimental conditions used here, does not seem to be able to inhibit
lipase production, but it does inhibit the enzyme activity because of its presence in the mixture of the reaction. |
| |
Keywords: | Lipase sodium dodecyl sulfate Candida lipolytica growth |
本文献已被 SpringerLink 等数据库收录! |
|