DNA interaction with synthetic polymers in solution |
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| Authors: | Nina Kasyanenko Daria Afanasieva Boris Dribinsky Dmitry Mukhin Olga Nazarova Evgeny Panarin |
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| Affiliation: | (1) Department of Molecular Biophysics, Faculty of Physics, St. Petersburg State University, Uluanovskaya St., 1, Petrodvorets, St.-Petersburg, 198504, Russia;(2) Institute of Macromolecular Compounds RAS, St. Petersburg, Russia |
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| Abstract: | DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N-trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made. |
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| Keywords: | DNA-polymeric complexes DNA condensation Polyvinylamine Polyallylamine Polydimethylaminoethylmethacrylate Polytrimethylammonioethyl methacrylate Poly- font-variant:small-caps" >l-lysine |
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