| 蒙药苘麻子中多糖的提取纯化及含量测定 |
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| 引用本文： | 赵玉英,司志鑫,刘飞,李敬朋,青松,陈伟.蒙药苘麻子中多糖的提取纯化及含量测定[J].光谱实验室,2010,27(4):1466-1468. |
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| 作者姓名： | 赵玉英 司志鑫 刘飞 李敬朋 青松 陈伟 |
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| 作者单位： | 内蒙古民族大学分析测试中心,内蒙古通辽市霍林河大街22号,028043 |
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| 基金项目： | 国家自然科学基金资助项目，内蒙古自然科学基金资助项目 |
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| 摘 要： | 采用热水提取-乙醇沉淀法从苘麻子中提取多糖,Sevag法除蛋白,苘麻子多糖经大孔树脂分离、透析得两种多糖,即QMZP-1和QMZP-2,采用分光光度法(λ=486nm)测定两种多糖含量,分别为1.8%和0.75%,求得校准曲线的回归方程为y=0.00435x+0.561,相关系数为r=0.9998,平均回收率为100.1%,RSD=0.71%(n=5)。
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| 关 键 词： | 多糖 苘麻子 纯化 |
Determination and Purification of Polysaccharide from Qingmazi |
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| ZHAO Yu-Ying,SI Zhi-Xin,LIU Fei,LI Jing-Peng,QING Song,CHEN Wei.Determination and Purification of Polysaccharide from Qingmazi[J].Chinese Journal of Spectroscopy Laboratory,2010,27(4):1466-1468. |
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| Authors: | ZHAO Yu-Ying SI Zhi-Xin LIU Fei LI Jing-Peng QING Song CHEN Wei |
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| Institution: | (Inner Mongolia Nationalities University,Tongliao,Inner Mongolia 028043,P.R.China) |
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| Abstract: | Polysaccharide was extracted from qingmazi with hot water-ethanol deposit method and wiped off deproteination with Sevag method. Polysaccharide of qingmazi was further purified by resin obtained QMZP-1,QMZP-2,and its content was determined by spectrophotometry（λ=486nm）.The regression equation is y=0.00435x＋0.561 and coefficient correlation is 0.9998.The average recovery was 100.1%,RSD=0.71%（n=5）. |
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| Keywords: | Polysaccharide Qingmazi Purification |
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