高效毛细管电泳法分析蓝色签字笔字迹色痕种类及相对形成时间

选用毛细管区带电泳分离模式,对13种市售蓝色签字笔样品字迹色痕进行了分析。当电压为15 kV、柱温为25 ℃时,以0.03 mol/L 四硼酸钠水溶液(外加10%(体积分数)的甲醇)为缓冲液,所有样品都可以得到良好的区分;依据紫外区检测结果,样品可以分为三大类、五小类;依据可见区检测结果,样品可以分为四类；两种分析结果相结合有助于增大不同样品之间的区分度;选择3种样品进行暗处保存老化制样,根据字迹中色料成分相对含量的变化,对字迹的相对形成时间进行了初步探讨。研究还发现纸张类型对于上述研究结果无影响。

Differentiation of Species of Blue Inks for Roller-Ball Pen and Determination of Relative Writing Ages by High Performance Capillary Electrophoresis

Traces of 13 blue roller-ball pen inks were identified by high performance capillary electrophoresis (HPCE) with 0.03 mol/L borate and 10% (in volume) methanol as buffer at the voltage of 15 kV and the temperature of 25 degrees C. Samples could be classified into 3 major groups and 5 subgroups by UV detection results and 4 species by visible detection results. The analytical results with different wavelength lights could be used to cross confirm the ink species. The aging effect was studied for 3 samples stored in darkness, and the relative ages were determined based on the relative quantities of some components. In addition, no effect of the matrix of paper was found on the results.