| HPLC法测定鲜食葡萄采后贮藏过程中糖分的变化 |
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| 引用本文： | 林江丽,朱亚娟,王金霞,封顺,王吉德.HPLC法测定鲜食葡萄采后贮藏过程中糖分的变化[J].新疆大学学报(理工版),2014(3):258-260. |
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| 作者姓名： | 林江丽 朱亚娟 王金霞 封顺 王吉德 |
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| 作者单位： | 新疆大学化学化工学院,新疆石油天然气精细化工教育部和自治区重点实验室 |
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| 基金项目： | 国家自然科学基金项目资助(31160341) |
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| 摘 要： | 利用高效液相色谱法测定鲜食葡萄采后贮藏过程中糖分的变化.色谱柱为Inertsil NH2柱,流动相为75%的乙腈水溶液,用示差折光检测器检测不同贮藏期鲜食葡萄果实中的糖分.本方法的相对标准偏差为0.52%～1.22%,相关系数分别为0.997 3和0.999 3,加标回收率为99.81%～100.4%.该方法简便、灵敏、结果可靠,两种糖都得到了较好的分离,适用于鲜食葡萄果实中糖分的分析.
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| 关 键 词： | HPLC 葡萄 贮藏 糖分 |
Determination of Grape Saccharide Components in Storage Time by High Performance Liquid Chromatography |
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| LIN Jiang-li;ZHU Ya-juan;WANG Jin-xia;FENG Shun;WANG Ji-de.Determination of Grape Saccharide Components in Storage Time by High Performance Liquid Chromatography[J].Journal of Xinjiang University(Science & Engineering),2014(3):258-260. |
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| Authors: | LIN Jiang-li;ZHU Ya-juan;WANG Jin-xia;FENG Shun;WANG Ji-de |
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| Institution: | LIN Jiang-li;ZHU Ya-juan;WANG Jin-xia;FENG Shun;WANG Ji-de;College of Chemistry and Chemical Engineering, Xinjiang University, Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education & Xinjiang Uyghur Autonomous Region; |
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| Abstract: | Determination of saccharide components in table grape was studied by high performance liquid chromatography（HPLC）.The HPLC column is Inertsil NH2, 5 μm, 4.6×250 mm,the mobile phase is CH3CN： H2O=75：25, and the detector is series RID-10 A refractive index detector.The relative standard deviations are 0.52%～1.22%, and recoveries ranged from 99.81～100.4%, while the linear related coefficients are 0.997 3 and 0.999 3, respectively.The results showed that the samples are separated completely.The method is proved to be simple,rapid and reliable to determine the saccharine components in table grape. |
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| Keywords: | HPLC grape storage saccharide |
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