Use of the modified quick easy cheap effective rugged and safe sample preparation approach for the simultaneous analysis of type A- and B-trichothecenes in wheat flour |
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Authors: | I Sospedra J BlesaJM Soriano J Mañes |
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Institution: | Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av.Vicent Andrés Estellés s/n, 46100 Burjassot, Spain |
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Abstract: | A suitable extraction and purification method for the simultaneous liquid chromatography–mass spectrometry (LC–MS) determination of five mycotoxins, three type A, diacetoxyscirpenol (DAS), T-2 toxin (T-2) and HT-2 toxin (HT-2), and two type B-trichothecenes, deoxynivalenol (DON) and nivalenol (NIV), has been optimised using a modified “Quick Easy Cheap Effective Rugged and Safe” (QuEChERS) method. Different solvents were studied in the extraction procedure to obtain better recoveries, which ranged from 86 to 108%, using a 85/15 (v/v) mixture of methanol/acetonitrile. The values obtained for recovery, repeatability and reproducibility of the optimized method are in agreement with Commission Directive 2005/26/EC for methods of analysis of Fusarium toxins. Finally, this optimized procedure was applied in wheat flour samples commercialized in Spain. |
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Keywords: | Trichothecenes QuEChERS Wheat flour LC&ndash MS |
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