Counter-current chromatographic separation of nucleic acid constituents with a hydrophilic solvent system |
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| Authors: | Yoichi Shibusawa Atsushi Shoji Chihiro Suzuka Akio Yanagida Yoichiro Ito |
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| Affiliation: | 1. Division of Pharmaceutical and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan;2. Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajyousui, Setagaya-ku, Tokyo 156-8550, Japan;3. Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA |
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| Abstract: | ![]()
Nucleic acid constituents such as nucleobases, nucleosides and nucleotides were separated by counter-current chromatography using type J coil planet centrifuge. The separation was performed with a hydrophilic solvent system composed of 1-propanol/800 mM potassium phosphate buffer (pH 7.4) (1:1, v/v) by eluting the lower aqueous phase at a flow-rate of 0.5 ml/min. Eight selected nucleic acid constituents (4.0 mg, 0.5 mg of each), uridine monophosphate (UMP), adenosine monophosphate (AMP), deoxyadenosine monophosphate (dAMP), uridine, urasile, deoxy uridine, adenosine and adenine were well resolved within 160 min. |
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| Keywords: | Counter-current chromatography Nucleic acid constituents Hydrophilic solvent system Type J coil planet centrifuge |
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