pH值对多类人地贫红细胞作用的拉曼光谱研究

地中海贫血是一种发病率高，影响大的血液疾病。以往对地中海贫血的检测方法繁琐，耗时较长。通过对酸、碱pH环境下不同种类地贫红细胞和正常红细胞拉曼光谱的对照，发现在酸性条件下，α-地贫和β-地贫的拉曼谱具有不同的特征：表征氧合血红蛋白的特征峰谱带在β-地贫下强度升高甚至完全取代脱氧血红蛋白的特征峰;β-地贫红细胞膜的链间侧向相互作用序参数较正常红细胞膜的小，且其S_lat值与正常相比减小的比例随贫血程度的不同而不同，而α-地贫的S_lat值与正常红细胞膜的值相当。结果显示，可以基于pH作用的拉曼光谱测定方法，用于协助对地中海贫血的快速诊断。

Raman Spectra of Different Kinds of Thalassemia Erythrocytes with the Effect of pH

Thalassemia is a kind of blood diseases which has high morbidity and large influence. Previous methods for diagnosis are all very cumbersome and time consuming. By comparing Raman spectra of different kinds of thalassemia and normal erythrocytes at acid or alkaline pH, it was found that β-thalassemia and α-thalassemia erythrocytes have dissimilar Raman spectra in the acidic environment, such as the Raman spectra of β-thalassemia erythrocytes showed higher intensity at the characteristic bands assigned to oxyhemoglobin, and the characteristic bands assigned to deoxyhemoglobin were even completely replaced; β-thalassemia erythrocytes membrane has a smaller chain interaction between the transverse order parameters than normal erythrocytes, and the S_lat values are different for different stages of anemia, while the S_lat values are similar between α-thalassemia and normal erythrocytes, indicating that based on the effect of pH it is possible to diagnose thalassemia more quickly by using Raman spectra.