基于微波法制备纳米银胶的三种病菌的SERS光谱初步研究

使用激发光为785 nm的便携式拉曼光谱仪分别对赤星病菌、谷镰刀病菌和香蕉炭疽悬浮液进行了普通拉曼光谱和表面增强拉曼散射(SERS)光谱检测。实验结果显示，微波法制备的纳米银胶对三种植物病菌均具有较好的增强效果，同时获得了三种病菌信噪比较好的SERS光谱。从整体上看，三种菌谱峰峰强分布具有一定的相似性，如在481 cm-1处均为最强谱峰，500～1 000 cm-1谱峰较弱和1 000～1 600 cm-1谱峰较强。但三者在谱峰的分布和峰形上仍有明显不同，因此通过比较三种病菌的不同SERS谱峰可对其进行快速区分和鉴别。

Elementary SERS Spectroscopy Studies of Three Kinds of Pathogens in Ag Colloids Prepared by Microwave Method

The normal Raman (NR) and surface enhanced Raman spectroscopy (SERS) for Alternaria alternate, Colletotrichum musae, and fusarium suspensions were measured by a portable Raman spectrometer (785 nm). The result indicates that the silver colloid prepared by microwave method shows high enhancement of Raman scattering for the three kinds of pathogens. Comparing SERS spectrums of the three kinds of pathogens, there are some similarities. In these SERS spectrums, the peaks in the range of 500~1 000 cm-1 are very weak. Additionally, the peaks in the range of 1 000~1 600 cm-1 are strong. Meanwhile, the peak at 481 cm-1 is the strongest. Although these spectrums are similar, there are some differences such as the distribution and shape of peaks. Therefore, the three kinds of pathogens can be discriminated quickly.