Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS |
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Authors: | Shelley?N?Jackson Hay-Yan?J?Wang Email author" target="_blank">Amina?S?WoodsEmail author Michael?Ugarov Thomas?Egan J?Albert?Schultz |
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Institution: | Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland 21224, USA. |
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Abstract: | After water, lipids are the most common biomolecules found in the brain (12%). A brief perusal of the physiology, anatomy,
and pathophysiology of the brain illustrates the importance of lipids. Recent advances in mass spectrometry have allowed the
direct probing of tissues. However, most studies have focused on proteins. In the present work, matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOFMS) and MALDI-ion mobility (IM)-TOFMS were employed for direct analysis of phospholipids
in rat brain tissue. Molecular ions (MH+) corresponding to phosphatidylcholines, phosphatidylethanolamines, and sphingomyelin, were recorded. When studying pharmacology,
we learn that many therapeutic compounds are stored in the body’s adipose tissue. MALDI-TOFMS and MALDI- IM-TOFMS were thus
used to analyze rat brain tissue with chlorisondamine added directly onto the tissue slice. With both techniques, noncovalent
complexes between the tissue phospholipids and chlorisondamine were detected. In addition, MALDI-IM-TOFMS of noncovalent complexes
between phospholipids and chlorisondamine displayed a mobility between that of an isobaric lipid and peptide. |
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