Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study |
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| Authors: | Dai Chen Xiao Wei Liang Yan Xie Lin Wang Guangji Ding Gang Meng Zhaoqing Zhang Juan Guan Tianye Kang An Zheng Xiao Xie Tong Li Chunzhu Zhao Qijin Liu Wenjun Zhao Li Xu Jia |
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| Institution: | Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, No. 24, Tongjiaxiang Street, Nanjing, 210009, China. |
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| Abstract: | A simple, sensitive and specific high‐performance liquid chromatography mass spectrometry (LC‐MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid–liquid extraction with ethyl acetate were separated on a C18 column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0 ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date. Copyright © 2011 John Wiley & Sons, Ltd. |
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| Keywords: | strictosamide LC‐MS pharmacokinetic study |
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