离子结合物在二茂铁介体电流型葡萄糖生物传感器中的应用研究

二茂铁及其衍生物由于氧化还原可逆性好,不溶于水,因此可利用其作介体电流型生物传感器的电子转移介体。但该类传感器的重现性、稳定性差。本文首次提出以含(C_6H_5)_4·(CH_3)_4离子缔合物的碳糊电极为基础电极,以二茂铁(FeCp_2)为电子转移介体制成葡萄糖生物传感器。该传感器重现性、稳定性好,响应迅速,测量准确度高。

Studies on Application of Ion Associate to Amperometric Biosensors for Glucose Based on Paste Modified by FeCp_2

This paper describes the design and testing of amperometric glucose biosensor in whichelectrical communication between the flavin redox centers of glucose oxidase and a conventionalcarbon paste electrode containing (C₆H₅).1B · N⁺(CH₃)₁ has been achieved by using electron-transfer relay system based on ferrocene (FeCp₂).Characteristics of the biosensor has been shown by cyclic voltammetry and stationary potential measurements.The biosensor responds rapidly to glucose, with steady-state current responsed achieved in less than 50 s.The response to glucose under N₂ saturation showed apparent Michcells-Menten constant, K_M^app, 21 mmol/L.The biosensor can be operated in a potential range where the oxidation of common interfering species, such as V_c and uric acid does not occur.Life of the biosensor is 50 days and plasma glucose assays were correlated with spectropllotometric determinations(Y=10.04 + 0.886X, r=0.99, n=9).