| 云南昭通产天麻中天麻多糖含量的测定 |
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| 引用本文： | 朱培芳,陈涛,顾雯,赵荣华.云南昭通产天麻中天麻多糖含量的测定[J].光谱实验室,2013(6):2960-2964. |
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| 作者姓名： | 朱培芳 陈涛 顾雯 赵荣华 |
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| 作者单位： | [1]云南中医学院实验中心,昆明市呈贡新城雨花路1076号650500 [2]云南中医学院中药学院,昆明市呈贡新城雨花路1076号650500 |
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| 基金项目： | 云南省科技2010年云南省社会发展科技计划项目（项目编号：2010CG022） |
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| 摘 要： | 建立了用苯酚-硫酸法测定云南昭通产天麻中天麻多糖的方法,实验结果表明：该法用于天麻多糖的含量测定时,供试品溶液在180min内显色稳定,RSD=0.351％（n=6）；回收率在97.36％-100.88％之间,平均回收率为99.25％,RSD=1.447％（n=6）.本实验结果可靠,并且该法不受蛋白质的影响.通过回收率实验可知,本法所测多糖含量,准确率较高,重复性较好,且此法简便,灵敏.为其质量标准的研究提供实验依据,并为天麻多糖的进一步研究奠定了基础.
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| 关 键 词： | 苯酚-硫酸法 云南昭通 天麻 多糖 |
Determination of Polysaccharide in Yunnan Zhaotong Gastrodia elata Bl.by Phenol-Sulfuric Acid Method |
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| ZHU Pei-Fang,CHEN Tao,GU Wen,ZHAO Rong-Hua.Determination of Polysaccharide in Yunnan Zhaotong Gastrodia elata Bl.by Phenol-Sulfuric Acid Method[J].Chinese Journal of Spectroscopy Laboratory,2013(6):2960-2964. |
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| Authors: | ZHU Pei-Fang CHEN Tao GU Wen ZHAO Rong-Hua |
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| Institution: | (Experimental center, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P. R. China;a De pt. of Traditional Ch inese Medicine, Yannan University of Traditional Chinese Medicine, Kunming 650500, P. R. China ) |
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| Abstract: | The method for determination of polysaccharide in Yunnan Zhaotong Gastrodia data BI. by phenol-sulfuric acid method was established. The test solution was stable in 180min, RSD= 0. 351%（n= 6）. The wavelength of UV detector was set at 488nm. The average recovery rate was 99. 250%, RSD=1. 447%（n = 6）. This method is rapid, accurate, and high in precision, good stability. |
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| Keywords: | Phenol-Sulfuric Acid Method Yunnan Zhaotong Gastrodia elata B1 Polysaecharide |
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