| 微孔板发光二极管诱导荧光-表面活性剂增敏法测定盐酸小檗碱 |
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| 引用本文： | 张雯,陈缵光,冯军,刘翠.微孔板发光二极管诱导荧光-表面活性剂增敏法测定盐酸小檗碱[J].分析化学,2011,39(8):1218-1222. |
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| 作者姓名： | 张雯 陈缵光 冯军 刘翠 |
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| 作者单位： | 中山大学药学院,广州,510006 |
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| 基金项目： | 国家自然科学基金(Nos.20727006,21075139); 中央高校基本科研业务费专项资金; 广东省科技计划项目(No.2008A030102009)资助项目 |
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| 摘 要： | 研制了微孔板发光二极管诱导荧光分析仪,以发光二极管为激发光源,Y形光纤束传导激发光和荧光,采用平面型光敏二极管接收荧光.采用二维定位器移动微孔板,可实现对多个样品快速连续的测定.利用弱碱性介质中阴离子表面活性剂十二烷基苯磺酸钠(SDBS)对盐酸小檗碱荧光的增敏作用,在pH 9.0的Britton-Robinson缓冲溶...
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| 关 键 词： | 发光二极管诱导荧光 盐酸小檗碱 表面活性剂增敏 十二烷基苯磺酸钠 |
Determination of Berberine Hydrochloride by Light-emitting Diode Induced Fluorescence Microplate Analyzer with Surfactant Sensitization |
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| ZHANG Wen,CHEN Zuan-Guang,FENG Jun,LIU Cui.Determination of Berberine Hydrochloride by Light-emitting Diode Induced Fluorescence Microplate Analyzer with Surfactant Sensitization[J].Chinese Journal of Analytical Chemistry,2011,39(8):1218-1222. |
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| Authors: | ZHANG Wen CHEN Zuan-Guang FENG Jun LIU Cui |
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| Institution: | ZHANG Wen,CHEN Zuan-Guang*,FENG Jun,LIU Cui(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006) |
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| Abstract: | A light-emitting diode induced fluorescence microplate analyzer was presented by using a light-emitted diode as excitation source,a planar photodiode as detector.The excitation light and fluorescence signal were transmitted by a Y-shape optical fiber bundle.All operation procedures of sample exchange could carry out by the movement of a 2-D localizer in a short time.In a Britton-Robinson buffer solution(pH 9.0),the fluorescence of berberine could be enhanced by the presence of anion surfactant.Under the exc... |
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| Keywords: | Light-emitting diode induced fluorescence Berberine hydrochloride Surfactant Sensitization Sodium dodecyl benzene sulfonate |
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