| 聚丙烯酸与核酸相互作用的共振光散射光谱研究及其分析应用 |
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| 引用本文： | 翟云云,王伟娟,曾延波,李蕾,路齐英,肖莹.聚丙烯酸与核酸相互作用的共振光散射光谱研究及其分析应用[J].分析科学学报,2011,27(3):359-362. |
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| 作者姓名： | 翟云云 王伟娟 曾延波 李蕾 路齐英 肖莹 |
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| 作者单位： | 嘉兴学院生物与化学工程学院;常州大学化学化工学院; |
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| 基金项目： | 浙江省科技计划重点项目(No.2007C23071); 浙江省自然科学基金(No.Y4080252) |
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| 摘 要： | 研究了聚丙烯酸(PAA)与脱氧核糖核酸(DNA)相互作用的共振光散射(RLS)光谱.实验结果表明,在pH=2.0的B-R缓冲溶液中,PAA与DNA自身的共振光散射峰均较弱,但当二者发生静电作用形成复合物后,体系的共振光散射峰增强,散射增强程度则各不相同,其相对散射强度的顺序是fsDNA＞ctDNA＞yRNA.在一定范围...
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| 关 键 词： | 共振光散射 聚丙烯酸 核酸 |
Study on the Interaction between Polyacrylic Acid and Nucleic Acid by Resonance Light Scattering Spectra and Its Analytical Application |
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| ZHAI Yun-yun,WANG Wei-juan,ZENG Yan-bo,LI Lei,LU Qi-ying,XIAO Ying.Study on the Interaction between Polyacrylic Acid and Nucleic Acid by Resonance Light Scattering Spectra and Its Analytical Application[J].Journal of Analytical Science,2011,27(3):359-362. |
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| Authors: | ZHAI Yun-yun WANG Wei-juan ZENG Yan-bo LI Lei LU Qi-ying XIAO Ying |
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| Institution: | ZHAI Yun-yun1,WANG Wei-juan2,1,ZENG Yan-bo1,LI Lei*1,2,LU Qi-ying2,XIAO Ying2,1 (1.College of Biology and Chemical Engineering,Jiaxing University,Jiaxing 314001,2.College of Chemistry and Chemical Engineering,Changzhou University,Changzhou 213016) |
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| Abstract: | A method based on the resonance light scattering(RLS) of PAA-DNA complex in Britton-Robinson buffer solution of pH=2.0 was proposed for the determination of DNA.The influences of some experimental factors,including pH value,PAA concentration,the sequence of adding the reagents and the coexistent substances,on the enhancement of the RLS intensity were studied.The RLS signal of PAA or nucleic acid alone was very weak,but it was greatly enhanced when they interacted with each other to form a bound product.Ther... |
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| Keywords: | Resonance light scattering Polyacrylic acid(PAA) DNA |
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