| 微透析活体取样与高效液相色谱-电化学检测法联用测定帕金森大鼠脑中神经递质 |
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| 引用本文： | 宋才勇,余莉,李雪艳,方亮莲,陈瑶如,高红昌,林丽. 微透析活体取样与高效液相色谱-电化学检测法联用测定帕金森大鼠脑中神经递质[J]. 分析试验室, 2011, 30(8): 80-83 |
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| 作者姓名： | 宋才勇 余莉 李雪艳 方亮莲 陈瑶如 高红昌 林丽 |
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| 作者单位： | 温州医学院药学院,温州,325035 |
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| 基金项目： | 浙江省自然基金项目(Y2100488); 浙江省教育厅科研项目(Y200906503); 浙江省大学生科技创新活动计划(2009R413051)资助 |
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| 摘 要： | 建立了一种微透析活体取样与高效液相色谱-电化学检测法联用技术测定自由活动大鼠脑中7种单胺类神经递质的方法.高效液相色谱采用Aglient XDB-C18柱,流动相为pH 3.0的0.1 mol/L H3PO4-NaH2PO4缓冲液与甲醇的混合液(90:10,V/V),流速为0.3 mL/min,电化学检测的工作电极为玻...
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| 关 键 词： | 微透析活体取样 高效液相色谱-电化学检测 单胺类神经递质 帕金森病 |
Determination of monoamine neurotransmitters in the striatum of parkinsonian rats by in vivo microdialysis coupled with high performance liquid chromatography-electrochemical detection |
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| SONG Cai-yong,YU Li,LI Xue-yan,FANG Liang-lian,CHEN Yao-ru,GAO Hong-chang,LIN Li. Determination of monoamine neurotransmitters in the striatum of parkinsonian rats by in vivo microdialysis coupled with high performance liquid chromatography-electrochemical detection[J]. Chinese Journal of Analysis Laboratory, 2011, 30(8): 80-83 |
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| Authors: | SONG Cai-yong YU Li LI Xue-yan FANG Liang-lian CHEN Yao-ru GAO Hong-chang LIN Li |
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| Affiliation: | SONG Cai-yong,YU Li,LI Xue-yan,FANG Liang-lian,CHEN Yao-ru,GAO Hong-chang and LIN Li(School of Pharmacy,Wenzhou Medical College,Wenzhou 325035) |
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| Abstract: | In vivo microdialysis sampling coupled with high performance liquid chromatography-electrochemical detection was developed and applied to the determination of the monoamine neurotransmitters in the striatum of freely moving rats.The HPLC system was equipped with an Agilent Eclipse XDB-C18 column.The mobile phase was 0.1 mol/L H3PO4-NaH2PO4 buffer solution-methanol with pH 3.0(90:10,V/V).The flow rate of the mobile phase was 0.3 mL/min.The glassy carbon electrode was choosen as the working electrode and the ... |
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| Keywords: | In vivo microdialysis sampling High performance liquid chromatography-electrochemical detection Monoamine neurotransmitters Parkinson's disease |
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