Identification of Bioactive Compounds of Asparagus officinalis L.: Permutation Test Allows Differentiation among “Triguero” and Hybrid Green Varieties |
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Authors: | Cecilia Jimnez-Snchez Fabin Pedregosa Isabel Borrs-Linares Jesús Lozano-Snchez Antonio Segura-Carretero |
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Institution: | 1.Department of Analytical Chemistry, Granada University, Avda Fuentenueva s/n, 18071 Granada, Spain;2.Functional Food and Development centre (CIDAF), Health Science Technological Park, Avda del Conocimiento s/n, 18016 Granada, Spain;3.EECS Department, University of California, Berkeley, CA 94720-1776, USA; |
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Abstract: | In this study, we determined the phytochemical profile of the Spanish “triguero” asparagus landrace “verde-morado” (Asparagus officinalis L.), a wild traditional landrace, and the improved “triguero” HT-801, together with two commercial green asparagus varieties. For comparison, we used reverse-phase high-performance liquid chromatography coupled with diode array electrospray time-of-flight mass spectrometry (RP-HPLC-DAD-ESI-TOF/MS) followed by a permutation test applied using a resampling methodology valid under a relaxed set of assumptions, such as i.i.d. errors (not necessarily normal) that are exchangeable under the null hypothesis. As a result, we postulate that “triguero” varieties (the improved HT-801 followed by its parent “verde-morado”) have a significantly different phytochemical profile from that of the other two commercial hybrid green varieties. In particular, we found compounds specific to the “triguero” varieties, such as feruloylhexosylhexose isomers, or isorhamnetin-3-O-glucoside, which was found only in the “triguero” variety HT-801. Although studies relating the phytochemical content of “triguero” asparagus varieties to its health-promoting effects are required, this characteristic phytochemical profile can be used for differentiating and revalorizating these asparagus cultivars. |
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Keywords: | asparagus Asparagaceae HPLC mass spectrometry classification |
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