The shielding effect of nano TiO2 on collagen under UV radiation was investigated using UV spectrophotometry. The results indicate that the shielding of collagen under UV radiation in the presence of nano TiO2 is significant. For 2.5 wt.‐% of TiO2, there is a greater shielding effect of UV radiation than that for 0.5 wt.‐% of TiO2. In addition, this study gives hints that a novel tanning agent with a UV shielding function can be formulated by using nano TiO2.