| 脲和盐酸胍诱导的牛碳酸酐酶B的去折叠 |
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| 引用本文： | 王世祥,陈清法,张潭,罗海燕.脲和盐酸胍诱导的牛碳酸酐酶B的去折叠[J].分析科学学报,2011,27(1):31-34. |
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| 作者姓名： | 王世祥 陈清法 张潭 罗海燕 |
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| 作者单位： | 西北大学生命科学学院; |
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| 基金项目： | 陕西省自然科学基金(No.2001K10-G3-(3)) |
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| 摘 要： | 采用非变性聚丙烯酰胺凝胶电泳、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、高效凝胶排阻色谱和激光光散射光谱研究了脲和盐酸胍诱导的牛碳酸酐酶B的去折叠.实验结果表明,在脲和盐酸胍诱导的牛碳酸酐酶B的去折叠过程中,溶液中只含有单分子牛碳酸酐酶B和二分子牛碳酸酐酶B集聚体;二分子牛碳酸酐酶B集聚体是通过单分子牛碳酸酐酶B之间的疏水和...
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| 关 键 词： | 牛碳酸酐酶B 去折叠 脲 盐酸胍 |
Studies on the Unfolding of Bovine Carbonic Anhydrase B Induced by Urea and Guanidine Hydrochloride |
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| WANG Shi-xiang,CHEN Qing-fa,ZHANG Tan,LUO Hai-yan.Studies on the Unfolding of Bovine Carbonic Anhydrase B Induced by Urea and Guanidine Hydrochloride[J].Journal of Analytical Science,2011,27(1):31-34. |
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| Authors: | WANG Shi-xiang CHEN Qing-fa ZHANG Tan LUO Hai-yan |
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| Institution: | WANG Shi-xiang,CHEN Qing-fa,ZHANG Tan,LUO Hai-yan(College of Life Science,Northwest University,Xi'an 710069) |
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| Abstract: | The unfolding of bovine carbonic anhydrase B induced by urea and guanidine hydrochloride was studied by using non-denatured PAGE,size-exclusion chromatography,laser light-scattering and SDS-PAGE.The results showed that bovine carbonic anhydrase B existed in unimolecular or bi-molecular form in the denaturation solution.The result of SDS-PAGE showed that the bi-molecular bovine carbonic anhydrase B aggregate was formed from unimolecular bovine carbonic anhydrase B in solution through hydrophobic and electros... |
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| Keywords: | Bovine carbonic anhydrase B Unfolding Urea Guanidine hydrochloride |
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