| 荧光量子点免疫标记法检测炭疽芽孢杆菌 |
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| 引用本文： | 刘晓红,罗金平,田青,刘春秀,蔡新霞. 荧光量子点免疫标记法检测炭疽芽孢杆菌[J]. 分析化学, 2011, 39(2): 163-167. DOI: 10.3724/SP.J.1096.2011.00163 |
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| 作者姓名： | 刘晓红 罗金平 田青 刘春秀 蔡新霞 |
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| 作者单位： | 1. 中国科学院电子学研究所传感技术联合国家重点实验室,北京100190;中国科学院研究生院,北京100190
2. 中国科学院电子学研究所传感技术联合国家重点实验室,北京,100190 |
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| 基金项目： | 国家863项目(Nos.2009AA03Z411,2007AA03Z428); 国家科技重大专项(No.2008Z10003003-02); 中科院优秀奖资助项目(No.0913190260); 中科院知识创新项目“太湖水域藻类污染传感器关键技术研究”资助 |
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| 摘 要： | 建立了荧光量子点标记-免疫分析技术联用检测炭疽芽孢杆菌的方法.通过抗原抗体反应,结合生物素与亲和素间的特异性相互作用,将QDs特异性标记在炭疽芽孢杆菌上,并利用荧光显微镜和荧光分光光度计进行了验证.采用实验室自制的便携式荧光检测系统对标记QDs的炭疽芽孢杆菌样品进行定量检测.结果表明,在炭疽芽孢杆菌浓度在100～1×1...
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| 关 键 词： | 荧光量子点 炭疽芽孢杆菌 定量检测 特异性 |
Detection of Bacillus Anthracis Using Fluorescence Immunoassay with Quantum Dots Labels |
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| LIU Xiao-Hong,LUO Jin-Ping,TIAN Qing,LIU Chun-Xiu,CAI Xin-Xia. Detection of Bacillus Anthracis Using Fluorescence Immunoassay with Quantum Dots Labels[J]. Chinese Journal of Analytical Chemistry, 2011, 39(2): 163-167. DOI: 10.3724/SP.J.1096.2011.00163 |
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| Authors: | LIU Xiao-Hong LUO Jin-Ping TIAN Qing LIU Chun-Xiu CAI Xin-Xia |
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| Affiliation: | LIU Xiao-Hong1,2,LUO Jin-Ping1,TIAN Qing1,LIU Chun-Xiu1,CAI Xin-Xia*1,2 1(State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100190) 2(Graduate School of Chinese Academy of Sciences,Beijing 100190) |
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| Abstract: | A method for detecting Bacillus anthracis(B.anthracis) was constructed with indirect immunofluorescence technique using quantum dots(QDs) as fluorescent probes.B.anthracis could be labeled with QDs selectively,that was proved by fluorescence microscope and F4500 spectrofluoro-meter.The laboratory-made system including biosensor and device was utilized as detection apparatus.A linear relationship of the fluorescence intensity and log total count of B.anthracis was obtained with correlation coefficient of 0.9... |
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| Keywords: | Quantum dots Bacillus anthracis Quantitative detection Specificity |
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