宇佐美曲霉 L336酸性蛋白酶 发酵过程动力学研究

宇佐美曲霉栗色变种 L336是一株高产酸性蛋白酶的生产菌 ,采用转置培养法 ,对其发酵过程的 一些动力学特点进行了研究 ,结果表明 ,酸性蛋白酶是细胞利用内源营养从头合成的 ,属于非生长关联 型; 腺嘌呤是菌体生长的生长因子 ,代谢类似物对酶合成产生较大的抑制 ;而蛋白胨是酶合成的诱导因 子 ,发酵液溶氧水平较大地影响酶合成能力 . 本研究为其工业发酵工艺的构建提供了理论依据 .

Kinetic studies on acid protease fermentation process of Aspergillus usamii L336

Aspergillus usamii L3 3 6can produce high yield acid protease.By replacem ent culture m ethod, som e kinetic characters of its fermentation process were studied. The time course of this process m anifests a distinct feature which belongs to the type non-growth-associated productformation,and the acid protease producing activity em erges when growth is ceased due to the exhaustion of adenine-group growth substance.The enzyme synthesis will be interrupted by inhibitors such as 8-azaguanine and 8- azaadenine,and will be increased by increasing oxygen concerntration in ferm ental liquid.These facts illustrated the De Novo syntheses of the enzyme protein. The effectof peptone in inducing the enzyme is m uch m ore remarkable,protein hydrolyate such as bean cake and silkworm chrysalis are also more effective in inducing the enzym e. All the results above lay the theoretical foundation of Aspergillus usamii ferm entation technique.