Developing a genetically encoded green fluorescent protein mutant for sensitive light-up fluorescent sensing and cellular imaging of Hg(II) |
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| Authors: | Tao Jiang Daiping Guo Qian Wang Xin Wu Zhao Li Zhenhua Zheng Boyuan Yin Lin Xia Jixian Tang Wenxin Luo Ningshao Xia Yunbao Jiang |
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| Affiliation: | 1. Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China;2. State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China |
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| Abstract: | Hg(II) is well-known for quenching fluorescence in a distance dependent manner. Nevertheless, when we exposed the fluorophore of a green fluorescent protein (GFP) toward Hg(II), through H148C mutation, the GFP fluorescence could be “lighted up” by Hg(II) down to sub-nM level. The detection linear range is 0.5–3.0 nM for protein solutions at 8.0 nM. The GFPH148C protein displayed a promising selectivity toward Hg(II) and also the cellular imaging capacity. Spectra measurements suggested that the ground-state redistribution of protein contributed to the fluorescence enhancement, which was found not limited to Hg(II), and thus presented an opening for building a pool of GFP-based chemosensors toward other heavy metal ions. |
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| Keywords: | Green fluorescent protein-based sensor Mercury ion Fluorescence light-up Cellular imaging |
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