Functional graphene-gold nano-composite fabricated electrochemical biosensor for direct and rapid detection of bisphenol A |
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Authors: | Daodong Pan Yuanyuan Gu Hangzhen Lan Yangying Sun Huiju Gao |
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Institution: | 1. Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China;2. Department of Food Science & Nutrition of Ginling College, Nanjing Normal University, Nanjing 210097, China |
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Abstract: | In this research, the graphene with excellent dispersity is prepared successfully by introducing gold nanoparticle to separate the individual sheets. Various techniques are adopted to characterize the prepared graphene and graphene-gold nanoparticle composite materials. This fabricated new composite material is used as the support material to construct a novel tyrosinase based biosensor for detection of bisphenol A (BPA). The electrochemical performances of the proposed new enzyme biosensor were investigated by differential pulse voltammetry (DPV) method. The proposed biosensor exhibited excellent performance for BPA determination with a wide linear range (2.5 × 10−3–3.0 μM), a highly reproducible response (RSD of 2.7%), low interferences and long-term stability. And more importantly, the calculated detection limit of the proposed biosensor was as low as 1 nM. Compared with other detection methods, this graphene-gold nanoparticle composite based tyrosinase biosensor is proved to be a promising and reliable tool for rapid detection of BPA for on-site analysis of emergency BPA related pollution affairs. |
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Keywords: | Graphene-gold nanoparticle Tyrosinase based biosensor Differential pulse voltammetry Bisphenol A |
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