Multiplexed immunoassays for the analysis of breast cancer biopsies |
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Authors: | Nicole Schneiderhan-Marra Georg Sauer Cornelia Kazmaier Hsin-Yun Hsu Karin Koretz Helmut Deissler Thomas O Joos |
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Institution: | 1. NMI, Natural and Medical Sciences Institute, University of Tuebingen, Markwiesenstra?e 55, 72770, Reutlingen, Germany 2. Department of Obstetrics and Gynaecology, University of Ulm Medical School, Frauensteige 14, 89075, Ulm, Germany 3. Department of Pathology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany 4. EDI GmbH, Aspenhaustra?e 25, 72770, Reutlingen, Germany
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Abstract: | Within the last decade, protein microarray technology has been successfully used for the simultaneous quantification of target
proteins from minimal amounts of samples in basic and applied proteome research. The robustness and appropriate sensitivity
of these miniaturized assays have been demonstrated and thus the transfer to routine and high-throughput applications is now
possible. In this study, multiplexed bead-based sandwich immunoassays were used to determine the concentrations of 54 protein
analytes, including HER 2 and the estrogen receptor, from ultrasound-guided large-core needle biopsies (LCNBs) from breast
cancer patients. Expression levels for HER 2, estrogen receptors and progesterone receptors were also assessed by immunohistochemical
routine staining, performed in the clinic on corresponding biopsy samples. The high concordance of the data sets generated
with the bead-based protein arrays and by conventional immunohistochemical assessment of HER 2 and the estrogen receptor expressed
by breast cancer cells present in the biopsies was demonstrated. |
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