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Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA |
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| Authors: | Rawil F Fakhrullin Victor G Vinter Alsu I Zamaleeva Maria V Matveeva Roustem A Kourbanov Belayhun K Temesgen Dilara G Ishmuchametova Zinaida I Abramova Olga A Konovalova Myakzyum K Salakhov |
| Institution: | (1) Department of Biochemistry, Kazan State University, Kremlevskaya 18, 420008 Kazan, Russia;(2) Department of Optics and Nanophotonics, Kazan State University, Kremlevskaya 18, 420008 Kazan, Russia;(3) Department of Biology, Tatar State University of Humanities and Education, 420021 Kazan, Russia |
| Abstract: | We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance
frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(l-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface
of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(l-lysine)–DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect
the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human
immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental
results indicate high sensitivity and selectivity of the immunosensor.
In memoriam Prof. Victor G. Vinter |
| Keywords: | Quartz crystal microbalance Atomic force microscopy Antibodies to double-stranded DNA Systemic lupus erythematosus Bronchial asthma Immunosensor |
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