Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30 |
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Authors: | Zsolt Szengyel Guido Zacchi |
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Institution: | (1) Department of Chemical Engineering 1, Lund University, PO Box 124, S-221 00 Lund, Sweden |
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Abstract: | Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting
degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present
study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels’s mineral medium, supplemented with steam-pretreated
willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration
levels of acetic acid (0–3.0 g/L) and furfural (0–1.2 g/L) were applied alone as well as in certain combinations. Two enzyme
activities, cellulase and β-glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was
1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained
without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity
even at the highest concentration. However, β-glucosidase activity was increased with increasing acetic acid concentration.
Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and β-glucosidase production. |
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Keywords: | Trichoderma reesei RUT C30 cellulase steam-pretreated willow inhibition acetic acid furfural |
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