T(4-Mop)PS4卟啉与牛血清蛋白相互作用的研究

以四-(4-甲氧基-3-磺酸基苯)卟啉(T(4-Mop)PS4)为探针,通过T(4-Mop)PS4与牛血清白蛋白(BSA)的相互作用,建立了测定BSA的电化学分析方法。T(4-Mop)PS4的峰电流变化(ΔIp)与BSA在2.0×10-6～1.0×10-5mol.L-1范围内呈良好的线性关系,检出限为1.2×10-6mol.L-1;对5.0×10-6mol.L-1BSA平行测定8次,其相对标准偏差为2.0%,回收率为95%～104%。组氨酸、缬氨酸、苯丙氨酸、丝氨酸、异白氨酸、谷氨酰胺、苏氨酸等氨基酸对BSA的测定不产生干扰。采用紫外可见光度法、荧光光谱法和线性扫描伏安法(LSV)研究了T(4-Mop)PS4与BSA之间的相互作用,并测定了二者相互作用的结合常数和结合比。研究表明,T(4-Mop)PS4与BSA之间主要以疏水作用力结合,形成了1∶1的稳定复合物。

Study on the Interaction of T(4 Mop)PS4 with BSA

Using meso-terakis(4-methoxyl-3-sulfonatophenyl) porphyrin(T(4-Mop)PS4) as an electrochemical probe,a new electroanalytical method for the determination of bovine serum albumin(BSA) was established based on the interaction of BSA with T(4-Mop)PS4.A good linear relationship was obtained between the variation of peak current and concentration of BSA in the range of 2.0×10-6-1.0×10-5 mol·L-1.The limit of detection(LOD) was 1.2×10-6 mol·L-1 and the relative standard deviation(RSD,n=8) for 5.0×10-6 mol·L-1 BSA was 2.0%.The supramolecular complex of T(4-Mop)PS4 with BSA was studied by three different methods,including spectrophotometry,fluorometry and linear sweep voltammetric(LSV) methods.The binding ratio and binding constant of T(4-Mop)PS4 with BSA were obtained.The experimental data demonstrated that T(4-Mop)PS4 could form a 1 ∶ 1 stable supramolecular complex with BSA,and hydrophobic interaction is the main reaction between them.