Antibody Activation using DNA‐Based Logic Gates |
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Authors: | Brian M G Janssen Martijn van Rosmalen Lotte van Beek Dr Maarten Merkx |
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Institution: | Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, De Rondom 70, 5612 AP Eindhoven (The Netherlands) |
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Abstract: | Oligonucleotide‐based molecular circuits offer the exciting possibility to introduce autonomous signal processing in biomedicine, synthetic biology, and molecular diagnostics. Here we introduce bivalent peptide–DNA conjugates as generic, noncovalent, and easily applicable molecular locks that allow the control of antibody activity using toehold‐mediated strand displacement reactions. Employing yeast as a cellular model system, reversible control of antibody targeting is demonstrated with low nM concentrations of peptide–DNA locks and oligonucleotide displacer strands. Introduction of two different toehold strands on the peptide–DNA lock allowed signal integration of two different inputs, yielding logic OR‐ and AND‐gates. The range of molecular inputs could be further extended to protein‐based triggers by using protein‐binding aptamers. |
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Keywords: | antibodies aptamers DNA nanotechnology molecular computing peptide– oligonucleotide conjugates |
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