NMR实验参数对IBP-血浆相互作用个性化差异研究的影响

药物与血浆蛋白相互作用强弱是影响药物分布代谢与药效的关键因素之一. 本研究小组已报道用扩散加权谱、弛豫加权谱结合主成分分析（PCA）方法研究布洛芬（IBP）与血浆蛋白相互作用的个体差异性. 该文则研究核磁共振实验参数的设置对血浆与药物相互作用个体差异性研究的影响. 以对照血浆样品组与加入布洛芬血浆组为模型，改变扩散时间、梯度强度、回波时间这3种实验参数，采集了27套不同实验设置的扩散加权谱与10套不同回波时间的弛豫加权谱. 结果表明,扩散时间为0.1 s~0.14 s且梯度强度为1.52×10^-3 T/cm~1.90×10^-3 T/cm时采集的扩散加权谱或回波时间为70 ms~110 ms时采集的弛豫加权谱更适合用来研究血浆与布洛芬相互作用的个性化差异.

Effects of NMR Parameters on Study of Individual Variations of Plasma-Ibuprofen Interactions

The interactions between drug and plasma proteins play a major role in drug absorption, metabolism and its efficacy. Previous reports demonstrated that diffusion and relaxation-weighted NMR combined with principal components analysis (PCA) could be used to characterize individual variations of plasma-drug interactions. In this research, using plasma with/without drug ibuprofen (IBP) as model, the NMR parameters including diffusion time, gradient strength for diffusion-weighted NMR and echo time for relaxation-weighted NMR were optimized. The results showed that gradient strength of 1.52×10^-3 T/cm~1.90×10^-3 T/cm under diffusion time of 0.1 s~0.14 s for diffusion-weighted NMR and echo time of 70 ms~110 ms for relaxation-weighted NMR are optimal for characterizing individual variations of plasma-drug interactions.