Simultaneous determination of hydrochlorothiazide and losartan potassium in tablets by high-performance low-pressure chromatography using a multi-syringe burette coupled to a monolithic column |
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Authors: | María Antonieta Obando José Manuel Estela Víctor Cerdà |
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Institution: | (1) Analysis and Quality Department, Faculty of Pharmacy, Los Andes University, 5101 Mérida, Venezuela;(2) Department of Chemistry, Faculty of Sciences, University of the Balearic Islands, 07122 Palma de Mallorca, Spain |
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Abstract: | This contribution describes use of a separation method based on on-line coupling of a multisyringe flow system with a chromatographic
monolithic column for simultaneous determination of hydrochlorothiazide and losartan potassium in tablets. The system comprised
a multisyringe module, three low-pressure solenoid valves, a monolithic C18 column (25 mm × 4.6 mm i.d.), and a diode-array detector. The mobile phase was 10 mmol L−1 potassium dihydrogen phosphate (pH 3.1)-acetonitrile-methanol (65:33:2 v/v/v) at a flow rate 0.8 mL min−1. UV detection was carried out at 226 nm. The multi-syringe chromatographic (MSC) method with UV spectrophotometric detection
was optimized and validated. Results from validation were very good. The analysis time was about 400 s. The method was found
to be applicable to routine analysis of both compounds in tablets. The coupling of the monolithic columns with a multi-syringe
flow-injection analysis manifold provides an excellent and inexpensive tool to solve the separation problems without use of
HPLC instrumentation. |
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Keywords: | Flow-injection analysis (FIA) Multisyringe chromatography (MSC) Monolithic column Drug monitoring Losartan potassium Hydrochlorothiazide |
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