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| 新型镍离子亲和色谱介质的制备及其性能考察 | |
| 引用本文： | 徐莉莉,陈革豫,张腾,张忠旗,韩广,李乾,赵金礼,刘建利. 新型镍离子亲和色谱介质的制备及其性能考察[J]. 色谱, 2016, 34(3): 232-239. DOI: 10.3724/SP.J.1123.2015.10030 |
| 作者姓名： | 徐莉莉 陈革豫 张腾 张忠旗 韩广 李乾 赵金礼 刘建利 |
| 作者单位： | 1. 西部资源生物与现代生物技术教育部重点实验室, 西北大学生命科学学院, 陕西 西安 710069;2. 西安华澳丽康活性蛋白与多肽工程中心, 陕西 西安 710054 |
| 基金项目： | 国家自然科学基金项目(30070905,20872118);陕西省重点实验室基金项目(2010JS097);陕西省教育厅基金项目(08jk477). |
| 摘 要： | 以氯甲基化聚苯乙烯交联微球(氯球)为载体基质、N,N-二甲基甲酰胺(DMF)为溶剂、N,N-二异丙基乙胺(DIEA)催化,将合成的Lys(Boc)-OEt、氯乙酸乙酯连接到微球上。经水解使羧基裸露,与Ni2+螯合,制得新型镍离子亲和色谱介质。将其应用于His-Lys-Tyr三肽及Phe-His-Thr三肽纯化研究。以不含组氨酸的六肽作为杂质模型,用所制备的镍离子亲和介质进行分离。结果表明,所制备的镍离子亲和介质对两个含组氨酸的三肽具有很好的亲和作用,并实现了与不含组氨酸六肽的分离。 |
| 关 键 词： | 含组氨酸短肽 氯甲基化聚苯乙烯交联微球 镍离子亲和色谱介质 |
| 收稿时间： | 2015-10-20 |
Preparation and properties of new nickel ion affinity chromatography medium |
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| XU Lili,CHEN Geyu,ZHANG Teng,ZHANG Zhongqi,HAN Guang,LI Qian,ZHAO Jinli,LIU Jianli. Preparation and properties of new nickel ion affinity chromatography medium[J]. Chinese journal of chromatography, 2016, 34(3): 232-239. DOI: 10.3724/SP.J.1123.2015.10030 | |
| Authors: | XU Lili CHEN Geyu ZHANG Teng ZHANG Zhongqi HAN Guang LI Qian ZHAO Jinli LIU Jianli |
| Affiliation: | 1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China;2. Active Protein & Polypeptide Engineering Center of Xi'an Hua Ao Li Kang, Xi'an 710054, China |
| Abstract: | With the chloromethylated crosslinking polystyrene microspheres (chlorine ball) as the matrices, N,N-dimethylformamide (DMF) as the solvent, activated by N,N-diisopropylethylamine (DIEA), the synthesized Lys(Boc)-OEt and ethyl chloroacetate were coupled to the matrices. After hydrolysis, the carboxyl group was released and chelated with Ni2+, and a new nickel ion affinity chromatographic medium was prepared. The affinity medium was applied to purify the His-Lys-Tyr and Phe-His-Thr tri-peptides. The results showed that the peptides containing histidine were retained on the affinity medium and separated with other substances. |
| Keywords: | chloromethylated crosslinking polystyrene microspheres histidine-containing peptides nickel ion affinity chromatography medium |
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