| Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode |
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| 作者姓名： | Li Bo NIE Jian Rong CHEN Yu Qing MIAO Nong Yue HE |
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| 作者单位： | Li Bo NIE1，2，Jian Rong CHEN1，Yu Qing MIAO1，Nong Yue HE3 1 College of Chemistry and Life Science，Zhejiang Normal University，Jinhua 321004 2 Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province，Hunan University of Technology，Zhuzhou 412008 3 State Key Laboratory of Bioelectronics，Southeast University，Nanjing 210096 |
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| 基金项目： | 中国科学院资助项目;湖南省自然科学基金 |
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| 摘 要： | Nanoporous materials have been widely applied to biosensor investigation. Recently, Guo et al. have investigated the mesoporous materials modified carbon paste electrode for rapid cTnI (cardiac troponin I) detection with enhanced sensitivity1-3. However, …
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| 关 键 词： | 碳纳米棒 碳糊电极 金纳米颗粒 灵敏度 电气化学研究 |
| 收稿时间： | 2005-11-28 |
Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode |
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| Li Bo NIE Jian Rong CHEN Yu Qing MIAO Nong Yue HE.Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode[J].Chinese Chemical Letters,2006,17(6):795-798. |
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| Authors: | Li Bo NIE Jian Rong CHEN Yu Qing MIAO Nong Yue HE |
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| Abstract: | Colloid gold nanoparticle-based layer-by-layer amplification approach was applied to enhance the electrochemical detection sensitivity of DNA hybridization at carbon nanotube modified carbon paste electrodes (CNTPEs). Streptavidin was immobilized onto the surface of CNTPEs, and the conjugation of biotin labeled target oligonucleotides to the above immobilized streptavidin was performed, followed by the hybridization of target oligonucleotides with the gold nanoparticle-labeled DNA probe and then the layer-by-layer enhanced connection of gold nanoparticles, on which oligonucleotides complementary to the DNA probe were attached, to the hybridization system. The differential pulse voltammetry (DPV) signal of total gold nano- particles was monitored. It was found that the layer-by-layer colloidal gold DPV detection enhanced the sensitivity by about one order of magnitude compared with that of one-layer detection. One-base mismatched DNA and complementary DNA could be distinguished clearly. |
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| Keywords: | Carbon nanotubes carbon paste electrode gold nanoparticles layer-by-layer amplify- cation sensitivity |
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