Sensitive and selective determination of metabolically formed trans-dihydrodiols and phenols of benzo[a]pyrene in water and urine samples by HPLC with amperometric detection |
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Authors: | E Fischer G Henze and K L Platt |
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Institution: | (1) Institut für Anorganische und Analytische Chemie der Technischen Universit?t Clausthal, Paul-Ernst-Stra?e 4, D-38678 Clausthal-Zellerfeld, Germany, DE;(2) Institut für Toxikologie, Universit?t Mainz, Obere Zahlbacher Strasse 67, D-55131 Mainz, Germany, DE |
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Abstract: | A method has been developed to separate hydroxylated metabolites of the carcinogenic polycyclic aromatic hydrocarbon benzoa]pyrene,
i. e. trans-4,5-, 7,8-, 9,10-dihydrodiol and 1-, 3-, 7-, and 9-phenol, by HPLC with amperometric detection employing an isocratic
methanol/water eluent (70:30, v/v) containing 0.5 g/L sulfuric acid and 1 g/L lithium perchlorate. Compared with the usually
applied fluorescence (λex = 265 nm, λem = 460 nm) and ultraviolet (λ = 265 nm) detection, the amperometric technique is about 2–12 times more sensitive for the determination
of all metabolites investigated. The method was applied to the determination of the seven metabolites of benzoa]pyrene in
different water samples and in urine after solid-phase extraction (SPE). The results obtained by HPLC with amperometric detection
after SPE enrichment from an aqueous extract of a soil sample and from the urine of a rat intragastrically treated with benzoa]pyrene
agreed well with the values determined with fluorescence and/or UV detection.
Received: 20 December 1996 / Revised: 10 March 1997 / Accepted: 30 April 1997 |
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