| 高效液相色谱法同时测定多维元素片中9种水溶性维生素 |
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| 引用本文： | 田颖,黄挺,刘军,杨屹. 高效液相色谱法同时测定多维元素片中9种水溶性维生素[J]. 分析试验室, 2010, 29(11) |
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| 作者姓名： | 田颖 黄挺 刘军 杨屹 |
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| 作者单位： | 北京化工大学理学院,北京,100029;中国计量科学研究院化学所,北京,100013 |
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| 基金项目： | 国家质检总局食品安全监管专项(ASPAQ0705)项目资助 |
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| 摘 要： | 采用更简便的流动相体系,建立了高效液相色谱法同时测定多维元素片中9种水溶性维生素的快速分析方法。以酸水解与离心的方法处理样品,用C8柱分离,流动相A为0.1%三氟乙酸的水溶液,B为甲醇,梯度洗脱,二极管阵列检测器(DAD)检测。28min内实现了9种水溶性维生素的同时分离测定。各维生素线性关系、精密度、回收率均良好。并使用美国国家标准技术研究院(NIST)的SRM3280多维元素片标准物质对方法进行了确认,运用此法测定了市售多维元素片中的水溶性维生素含量。该法可作为维生素片剂中水溶性维生素分离测定的质控方法。
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| 关 键 词： | 高效液相色谱 水溶性维生素 多维元素片 |
Simultaneous determination of water-soluble vitamins in multivitamin tablets by high performance liquid chromatography |
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| TiAN Ying,HUANG Ting,LIU Jun,YANG Yi. Simultaneous determination of water-soluble vitamins in multivitamin tablets by high performance liquid chromatography[J]. Chinese Journal of Analysis Laboratory, 2010, 29(11) |
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| Authors: | TiAN Ying HUANG Ting LIU Jun YANG Yi |
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| Affiliation: | TIAN Ying1,HUANG Ting2,LIU Jun2 and YANG Yi1 (1.College of Science,Beijing University of Chemical Technology,Beijing 100029,2.National Institute of Metrology,National Research Center for CRMs,Beijing 100013) |
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| Abstract: | Using the optimized mobile phase system,a rapid,simple and accurate high performance liquid chromatographic method was developed and validated for the simultaneous determination of nine water-soluble vitamins in multivitamin tablets.The sample preparation involves an acid extraction of vitamins and two sample centrifugations.The separation was accomplished on a C8 column with methanol and 0.1% trifluoroacetic acid aqueous solution as mobile phase under gradient elution with diode array detection.Furthermore... |
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| Keywords: | high performance liquid chromatography water-soluble vitamins multivitamin tablets |
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