Spectroscopic determination of binding between human serum albumin and a platinum(II) dimethylsulfoxide complex |
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Authors: | K. R. Grigoryan M. G. Aznauryan N. A. Bagramyan L. G. Gevorkyan Sh. A. Markaryan |
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Affiliation: | (1) Yerevan State University, 1 ul. Al. Manukyan, Erevan, 0025, Armenia |
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Abstract: | We have used electronic absorption and fluorescence spectroscopy to study binding between a platinum(II) dimethylsulfoxide complex (cis-[Pt(DMSO)2Cl2]) and human serum albumin (HSA), and the effect of complexation on the structure of the protein. We have calculated the binding parameters for binding between cis-[Pt(DMSO)2Cl2] and HSA. We have determined the binding constant KB = (1.2 ± 0.1)·103 M−1 and the Hill coefficient h = 1.03 ± 0.1. We have determined that binding between cis-[Pt(DMSO)2Cl2] and the protein leads to a change in the internal packing of the macromolecule. __________ Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 75, No. 4, pp. 573–576, July–August, 2008. |
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Keywords: | human serum albumin cisplatin complexation UV spectroscopy fluorescence spectroscopy |
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