Novel phosphoryl derivatization method for peptide sequencing by electrospray ionization mass spectrometry |
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Authors: | Chen Jing Chen Yi Gong Peng Jiang Yang Li Yan-Mei Zhao Yu-Fen |
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Institution: | The Key Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, P.R. China. |
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Abstract: | A one-step phosphoryl derivatization method has been used in a peptide sequencing procedure for electrospray ionization tandem mass spectrometry (ESI-MS/MS). The sodiated derivatized peptides exhibit very simple dissociation patterns, in which two kinds of fragment ions, b(n) + OH + Na]+ and a(n) + Na]+, are formed. Since the amino acid residues are lost sequentially from the C-terminus, peptide sequences can be identified easily. The fragmentation efficiency of peptides increased as a result of the phosphorylation, and also provided peaks of useful intensity at lower m/z. A peptide with lysine at the C-terminus was derivatized and analyzed by ESI-MS/MS. Similar mass spectra, from which the sequence could be read out, were obtained. This is a novel derivatization method yielding neutral derivatives that should be suitable for peptide sequencing by LC/ESI-MS/MS. |
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