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Sensitive detection of tuberculosis using nanoparticle-enhanced surface plasmon resonance |
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| Authors: | Hongxia Chen Feng Liu Kwangnak Koh Jaebeom Lee Zonghuang Ye Tingting Yin Lizhou Sun |
| Affiliation: | 1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444, People’s Republic of China 2. Department of Applied Nanoscience, Pusan National University, Miryang, 627-706, Republic of Korea 3. Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People’s Republic of China 4. Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210036, China |
| Abstract: | We show that the antigen CFP-10 (found in tissue fluids of tuberculosis patients) can be used as a marker protein in a surface-plasmon resonance (SPR) based method for early and simplified diagnosis of tuberculosis. A sandwich SPR immunosensor was constructed by immobilizing the CFP-10 antibody on a self-assembled monolayer on a gold surface, this followed by blocking it with bovine serum albumin. Following exposure of the sensor surface to a sample containing CFP-10, secondary antibody immobilized on nickel oxide nanoparticles are injected which causes a large SPR signal change. The method has a dynamic range from 0.1 to around 150 ng per mL of CFP-10, and a detection limit as low as 0.1 ng per mL. This is assumed to be due to the high amplification power of the NiO nanoparticles. Schematic diagram of sensor chip configuration (left) and SPR study based on amplification strategy with NiO nanoparticles (right). |
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