Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization |
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Authors: | Sheng Liu Jinqing Wang Dong Zhang Junfei Ou Bin Liu |
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Institution: | a Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China b Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China c School of Stomatology, Lanzhou University, Lanzhou 730000, PR China |
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Abstract: | Considering for the potential application in tissue engineering, polyaniline (PANi) film was fabricated via a two-step route: a self-assembled monolayer of C6H5NHC3H6Si(OMe)3 was firstly formed on the single-crystal Si substrate; the conducting PANi film was then prepared through electroless surface polymerization of the aniline molecules on the aniline monolayer-bearing silane surface in an acidic aqueous solution. The formation of PANi film on Si surface was confirmed by characterizations of X-ray photoelectron spectroscope (XPS) and specular reflectance Fourier transform infrared (SR-FTIR) spectrum, etc. At last, the proliferation behaviors of PC-12 cells on the PANi film surface were studied by the 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) colorimetric assays, acridine orange fluorometric staining, and scanning electron microscope (SEM) observation, etc. The results demonstrate that the as-prepared PANi film provides high ability for cell proliferation, exhibiting promising potentials as surface coating to cultivate neuronal cells for applications in the tissue engineering. |
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Keywords: | Polyaniline film Electroless surface polymerization PC-12 cell Cell biocompatibility |
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